serum myoglobin

Serum myoglobin (Mb) is one of the earliest markers of elevation in patients with acute myocardial infarction (AMI). The blood concentration can be increased in 2 to 4 hours, and Mb is increased in almost all AMI patients in 6 to 10 hours, so Mb is positive. Especially helpful for the diagnosis of early AMI. It can be restored to normal within 24 hours of onset, so Mb negative is particularly helpful in eliminating the presence or absence of infarct expansion during the course of AMI. The immunoturbidimetric assay can be completed within 10 min. Basic Information Specialist classification: cardiovascular examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Rare. Normal value: Serum myoglobin: 12.7-45.3μg/L Above normal: Determination of Mb in serum is a sensitive indicator for early diagnosis of acute myocardial infarction; blood is taken from 12h after the onset of chest pain, and serum Mb is elevated. At this time, CK and AST are normal. The onset of the disease reached a peak 24 hours. Patients with pseudohypertrophic muscular dystrophy have elevated serum Mb, acute or chronic renal insufficiency, and severe congestive heart failure. negative: Positive: Tips: strenuous exercise, intramuscular injection, muscle trauma, cardiac catheterization, etc. can also increase myoglobin. Normal value 1. ELISA method 3.5 to 22.8 ng/ml. 2. RIA method healthy adult serum 29±16.3μg/L. Clinical significance 1. Determination of Mb in serum is a sensitive indicator for early diagnosis of acute myocardial infarction; blood is taken from 12h after the onset of chest pain, and serum Mb is elevated. At this time, CK and AST are normal. The onset of the disease reached a peak 24 hours. 2. The increase of serum Mb in patients undergoing cardiac surgery can be used as an important objective indicator for judging the degree of myocardial injury and healing. 3, in the clinical myopathy study found that patients with pseudo-hypertrophic muscular dystrophy increased serum Mb; up to 1150μg / L. 4, acute or chronic renal insufficiency, severe congestive heart failure, etc. also increased. High results may be diseases: myocardial infarction, congestive heart failure, pediatric pseudo-hypertrophic muscular dystrophy, dermatomyositis, renal insufficiency Strenuous exercise, intramuscular injection, muscle trauma, cardiac catheterization, etc. It can also increase myoglobin. Inspection process Immediately after blood collection, the test is sent for testing. 1. ELISA method: Take a clean and dry polypropylene microporous reaction plate, mark the blank, standard and determination, and proceed as follows: (1) Add 200 μl% normal rabbit serum to the blank control well, add 200 μl coating solution (containing Mb antibody) to each well, place at 37 ° C for 30 min, then set at 4 ° C overnight, discard the supernatant, and wash 3 times with washing solution. Leave it at room temperature for 3 min each time. (2) Add 100 μl of 14% normal human serum to the blank control well; add standard Mb solution of 5, 10, 20, 40, 80, 160, 320 ng/ml (prepared with diluent) 20 μl, and then add 4% normal. Human serum, 20 μl of sample serum was added to the assay well, and 8 μl of 4% normal human serum was added. 100 μl of the enzyme-labeled Mb solution was added to each well, and the solution was allowed to stand at 37 ° C for 1 h, and the solution was discarded and washed three times with the washing solution. (3) Add 200 μl of substrate solution to each well at 37 ° C for 15 min (protected from light). (4) 2 mol/L H2SO4 was added to each well to terminate the reaction, shaken for 1 min, left at room temperature for 10 min, zeroed with blank control wells, and the absorbance of each well was read at 492 nm. 2. RIA method: (1) Take 10×75mm plastic test tube, add 0.1ml of 20g/LBSA solution to zero standard tube, 0.1ml of standard tube (S1～4) plus standard solution, and measure 0.1ml of serum to be tested. 0.1 ml of antiserum was added to each tube, mixed and placed at 37 ° C for 20 min in a water bath. (2) Add 0.1 ml of 125I-Mb solution to each tube, mix, put in a 37 ° C water bath for 20 min, then add 0.5 ml of PR reagent to each tube, and continue to put in a 37 ° C water bath for 30 min. (3) Centrifugation (3500 r/min) for 15 min. The supernatant was discarded and radioactivity was measured on an automated gamma counter. (4) Taking B/B0% as the ordinate and the corresponding standard Mb concentration as the abscissa, draw a standard curve. The Mb concentration in the sample was found from the standard curve based on the B/B0% of the sample tube. Not suitable for the crowd Special diseases: Patients with hematopoietic dysfunction, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks Risk of infection: If you use an unclean needle, you may be at risk of infection.