Vitamin B1 (VitB1, VB1)

Vitamin B1 is also known as thiamine. The role of thiamine in the body is to participate in carbohydrate metabolism by carboxylase, a coenzyme of the hydroxyacetaldehyde system, which is a key material basis for material metabolism and energy metabolism. Vitamin B1 is also involved in the oxidative decarboxylation of the body and is essential for the metabolism of branched chain amino acids. In addition, vitamin B1 also plays an important role in promoting appetite, normal peristalsis of the gastrointestinal tract, and secretion of digestive juice. Vitamin B1 is mainly found in the outer skin and germ of seeds, such as rice bran and bran, which is abundant in yeast and abundant in yeast. Lean meat, cabbage and celery are also abundant. The vitamin B1 currently used is a chemically synthesized product. In the body, vitamin B1 participates in the catabolism of sugar in the form of coenzyme, which has the function of protecting the nervous system; it can also promote gastrointestinal motility and increase appetite. Basic Information Specialist classification: inspection classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not wear clothes that are too small or too tight, so as to avoid the sleeves being too tight when blood is drawn or the sleeves are too tight after blood drawing, causing blood vessels in the arms. Normal value Plasma: 0.039 μmol/L (1 μg/dl). Red blood cells: 0.23 to 0.46 μmol/L (6 to 12 μg/dl). White blood cells: 1.38 to 2.3 μmol/L (30 to 60 μg/dl). TPP effect method: 0 to 15% is normal. 15.1% to 25% vitamin B1 biochemical deficiency. >25% vitamin B1 is severely deficient. Clinical significance 1, vitamin B1 content: increased in oral or non-oral B1 preparations in excess. 2, vitamin B1 content decreased: seen in beriberi, Wernicke encephalopathy (cerebral beriberi syndrome), the potential B1 deficiency state. Low results may be diseases: beriberi considerations Taboo before the examination: It is necessary to cooperate with the doctor to write the correct name, neat and tidy, to avoid confusion caused by the same name or similar names. With these in mind, blood draws are more convenient and faster, and you can better save yourself time for diagnosis. Requirements for inspection: Do not wear clothes that are too small or too tight in cuffs to avoid the sleeves being too tight when blood is drawn or the sleeves are too tight after blood drawing, causing blood vessels in the arms. Different laboratory items should be asked by the doctor and treated differently. Inspection process Venous puncture, the venous blood is collected immediately after the test. ELISA procedure: coating with purified human, pig or bovine crystalline insulin, the test serum is diluted 1:10, plus the optimal amount of working concentration of HRP-labeled anti-human IgC antibody or labeled anti-human IgA F (ab '), add substrate color, positive results and then test the highest dilution of serum AIAb. METHODS: Purified human prothrombin concentration of 10 μg/ml was coated in high-activity-active polyvinyl chloride micropores, blocked with goat serum, and serum was titrated with Tirs hydrochloride buffered saline and containing 1% bovine serum albumin. :100 diluted, the second antibody is peroxidase-labeled goat anti-human IgG or IgM, the dilution is 1:10000, the tetramethylbenzidine as the substrate, 1mmol/L HCl as the stop solution established ELISA reaction system, Anti-prothrombin antibodies (aPT)-IgG and IgM in serum were detected. Not suitable for the crowd Inappropriate people: generally no special population. Adverse reactions and risks no.