saliva-rich histones

The rich histones in saliva are mainly derived from the parotid gland, submandibular gland, and sublingual gland. Its composition is characterized by a large amount of basic amino acids (about 48%) in the molecule, half of which are histidine, and these basic amino acids are closely related to their functions. A distinguishing feature of rich histone proteins, unlike other salivary proteins, is that their RNA is confined to the human parotid gland and submandibular gland, and it is speculated that its function is limited to saliva. Basic Information Specialist classification: oral examination classification: body fluid examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Rich histones have important preventive effects in the development of rickets, oral mucosal diseases, and periodontal diseases. When they are too low, they cannot prevent them. Normal value: Irritant saliva rich histone: 1-2nmol/ml Non-irritating saliva rich histone: 2-30nmol/ml Above normal: It helps to maintain the surface integrity of the enamel, inhibits the growth of fungi and the production of buds, which interferes with the colonization of the oral cavity and plays an important role in the elimination of oral cariogenic bacteria. negative: Positive: Tips: Oral contraceptives can reduce the results. Normal value Irritating saliva contains 1 to 2 nmol/ml. Non-irritating saliva contains 2 to 30 nmol/ml. Clinical significance 1. Helps maintain the integrity of the enamel surface. 2, inhibit the growth of fungi and the production of buds, thereby disturbing their settlement in the mouth. 3, low pH, nutrient deficiency, low ion concentration environment rich in histones antibacterial effect is stronger, has a certain cohesive effect on some streptococci, so it plays an important role in the removal of oral cariogenic bacteria. 4. Rich histones have important preventive effects in the development of caries, oral mucosal diseases and periodontal disease. Low result may be disease: caries note Oral contraceptives can reduce the results. Inspection process 1. Automatic rate turbidity turbidimetry: Take Beckman ICS-II as an example. Imported reagents can be used, or homemade reagents can be used. After calibration, use according to the operating instructions. Immunoglobulin G (IgG) is now taken as an example. (1) Dilute the sample to be tested to 1:36 or 1:216 with a computer diluter. (2) The IgG-corrected computer card and the IgG anti-serum computer card to be tested are inserted into the instrument successively, and the relevant test parameters and standard curves are automatically recorded in the microprocessor. (3) A reaction tube of 600 μl of the reaction solution with a stir bar was inserted into the reaction chamber. (4) The diluted sample to be measured and the IgG antiserum were separately injected into the reaction tube by a 42 μl sampler, and the IgG content was automatically displayed within 60 s through the display screen. 2, manual scattering turbidimetric assay: Because automatic reagents are subject to many restrictions, manual operation can replace automatic reagents, which is very important for the continued development of micro-protein assays. (1) Preparation of antigen: In order to determine the ratio of use of antiserum, the most suitable antigen content must be determined. The optimal detection range of ICS should be between 0.2 and 20 mg/L, and the optimum antigen midpoint concentration is 2 mg/L (refer to the final concentration in the reaction tube). The reference saliva was diluted with the dilution solution, set aside, and other concentrations were prepared in the same manner, and multiple points were selected to prepare a calibration curve. The sample should be clear and transparent, its background velocity value (RU) should not exceed 50, and the scattering value (SU) should not exceed 30. (2) Preparation of antiserum: strong specificity. The high-potency antiserum is appropriately diluted, and after filtering through a 0.45 μm microporous membrane, the optimal gain card (M11, M22, M33, M44) is selected, and the known amount of reference saliva is used as a standard antigen, and the method is manually The ICS-specific antiserum was applied to the same antigen as the self-made antiserum, and the rate unit (RU) was measured. The RU of the homemade antiserum was divided by the RU of the Beckman antiserum, which is the dilution factor of the homemade antiserum. (3) Preparation of reference curve: The known antigen is diluted to different concentrations and reacted with antiserum respectively. The measured RU value is the ordinate, and the corresponding antigen concentration is the abscissa and the curve to obtain the sample content. (4) Specimen determination: All body fluids can be measured. Generally, serum samples can be diluted according to the best detection concentration in the reaction tube. The cerebrospinal fluid (CSF) can be directly measured without dilution. The measured value of the RU value is multiplied by the dilution factor to obtain the specimen measurement value. After the above operation is completed, the sample measurement is completed, and the other steps are repeated 6 to 10 steps. The obtained rate value is checked for the calibration curve, that is, the unknown sample concentration is obtained. Not suitable for the crowd Those who take birth control pills should not be tested. Adverse reactions and risks There can be complications: 1, oral rickets, periodontitis. 2. Oral fungal infections.