salivary amylase

Salivary amylase (Salivary amylase) is a hydrolase secreted by the salivary glands. It is an enzyme that acts on α-1,4-glucan such as soluble starch, amylose, and glycogen to hydrolyze α-1,4-glycosidic bonds. . It belongs to one kind of α-amylase. AMS has two kinds of α and β, belonging to glycosidic chain hydrolase, and having a molecular weight of 4,000 to 50,000 D. Human AMS is α-type, mainly derived from pancreas and salivary glands. The proximal duodenum, lung, uterus, lactating breast and other organs are also secreted in small amounts. AMS plays an important role in the digestion of polysaccharides in food. Due to its small molecular weight, it is easily discharged from the urine. Basic Information Specialist classification: oral examination classification: body fluid examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Reduced in pancreatitis. Normal value: Salivary amylase: 607-3423U Above normal: Rare. negative: Positive: Reminder: The concentration of iodine solution can affect the measurement results. Generally, the iodine application solution should be reconstituted for more than 1 month. Normal value Normal value: 607 ~ 3423U. Clinical significance Reduced: seen in pancreatitis. Low results may be diseases: precautions for pancreatitis 1. Salivary and heparin anticoagulated plasma results were consistent, while EDTA-Na2, oxalate, citrate, and sodium fluoride inhibited AMS activity, resulting in low results. 2, the enzyme activity is significantly increased, that is, when the absorbance of the measuring tube is less than half of the absorbance of the blank tube, due to the relative shortage of the substrate, the specimen should be increased by the dilution factor, or the amount of the sample added, and the measurement result multiplied by the dilution factor. 3. AMS activity in saliva is very high and should be prevented from entering. 4. If the starch base liquid appears turbid or floc, suggesting deterioration or contamination, it should be reconstituted. Inspection process The saliva samples were collected and sent for examination, and detected by the iodine-starch colorimetric method. The starch in the matrix is ​​hydrolyzed by the alpha-amylase in the sample to produce glucose, maltose and dextrin. The remaining starch is combined with iodine solution to form a blue complex, and the color depth is inversely proportional to the enzyme activity. Not suitable for the crowd no. Adverse reactions and risks no.