salivary albumin

The proteins in saliva are mainly mucin, and other proteins are low in content. To date, more than ten kinds of salivary proteins and enzymes have been discovered as genetic markers, which have certain significance for human genetics and forensic research. Basic Information Specialist classification: oral examination classification: body fluid examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: normal. Normal value: Salivary albumin: 0-10mg/L Above normal: The blood saliva barrier is destroyed, plasma albumin protein enters saliva, and albumin increases. negative: Positive: Tips: Polyoxyethylene lauryl ether in reagents can also be replaced by other surfactants, such as Tween-20, in an amount of 2ml/L. Normal value Normal value salivary albumin <10mg/L. Clinical significance When the salivary glands are suffering from inflammation, tumors, etc., the blood saliva barrier is destroyed, plasma albumin proteins enter saliva, and albumin is increased. The increase in salivary albumin in patients with dry eye syndrome (Sjogren syndrome) is positively correlated with the degree of glandular destruction. High results may be diseases: Sjogren's syndrome precautions 1. When the albumin standard of 60g/L is combined with BCG, the solution light path is 1cm, and the absorbance at 630nm should be 0.811±0.035. If this value is not reached, the sensitivity is poor. 2. The inter-assay coefficient of variation of normal serum samples determined by this method is about 6.3%. 3. The polyoxyethylene lauryl ether in the reagent can also be replaced by other surfactants, such as Tween-20, in an amount of 2 ml/L. Inspection process 1. Automatic rate turbidity turbidimetry: Take Beckman ICS-II as an example. Imported reagents can be used, or homemade reagents can be used. After calibration, use according to the operating instructions. Immunoglobulin G (IgG) is now taken as an example. (1) Dilute the sample to be tested to 1:36 or 1:216 with a computer diluter. (2) The IgG-corrected computer card and the IgG anti-serum computer card to be tested are inserted into the instrument successively, and the relevant test parameters and standard curves are automatically recorded in the microprocessor. (3) A reaction tube of 600 μl of the reaction solution with a stir bar was inserted into the reaction chamber. (4) The diluted sample to be measured and the IgG antiserum were separately injected into the reaction tube by a 42 μl sampler, and the IgG content was automatically displayed within 60 s through the display screen. 2, manual scattering turbidimetric assay: Because automatic reagents are subject to many restrictions, manual operation can replace automatic reagents, which is very important for the continued development of micro-protein assays. (1) Preparation of antigen: In order to determine the ratio of use of antiserum, the most suitable antigen content must be determined. The optimal detection range of ICS should be between 0.2 and 20 mg/L, and the optimum antigen midpoint concentration is 2 mg/L (refer to the final concentration in the reaction tube). The reference saliva was diluted with the dilution solution, set aside, and other concentrations were prepared in the same manner, and multiple points were selected to prepare a calibration curve. The sample should be clear and transparent, its background velocity value (RU) should not exceed 50, and the scattering value (SU) should not exceed 30. (2) Preparation of antiserum: strong specificity. The anti-serum with high titer is appropriately diluted, and after filtering through a 0.45 μm microporous membrane, the optimal gain card (M11, M22, M33, M44) is selected, and the reference serum of known content is used as a standard antigen, and the method is manually The ICS-specific antiserum was applied to the same antigen as the self-made antiserum, and the rate unit (RU) was measured. The RU of the homemade antiserum was divided by the RU of the Beckman antiserum, which is the dilution factor of the homemade antiserum. The antiserum produced by the Shanghai Institute of Biological Products (batch No. 890201) was diluted with anti-IgG 1:15, diluted with anti-IgA 1:6, and diluted with anti-IgM 1:21. (3) Preparation of reference curve: The known antigen is diluted to different concentrations, respectively reacted with antiserum, and the measured RU value is the ordinate, and the corresponding antigen concentration is the abscissa and the curve. (4) Specimen determination: All body fluids can be measured. Generally, serum samples can be diluted according to the best detection concentration in the reaction tube. The cerebrospinal fluid (CSF) can be directly measured without dilution. The measured value of the RU value is multiplied by the dilution factor to obtain the specimen measurement value. 3. Manual rate scatter turbidimetry. After the above operation is completed, the sample measurement is completed, and the other steps are repeated 6 to 10 steps. The obtained rate value is checked for the calibration curve, that is, the unknown sample concentration is obtained. Not suitable for the crowd no. Adverse reactions and risks no.