Glycogen staining

The polysaccharide containing 1,2-ethanediol in the cell is oxidized by iodic acid to produce an aldehyde group, which acts as a special dye solution to change the colorless magenta into a red compound and precipitate in the cytoplasm. The depth of the red is proportional to the amount of 1,2-ethanediol groups that react in the cells. For the identification of atypical megakaryocytes and Li-S cells, the former PAS reaction is strongly positive, the latter is negative or weakly positive; used for the identification of high snow cells and Niemann-Pick cells, the former PAS reaction is strongly positive, The latter reaction is negative or weak. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normal; Hodgkin cells are weakly positive or negative. Positive: 1. Acute lymphocytic leukemia, lymphoid malignant proliferative diseases, erythroleukemia, Gaucher disease, primordial cells are strongly positive or positive; iron deficiency anemia, globin production disorders, myelodysplastic syndrome can also be positive Response; acute myeloid leukemia, acute monocytic leukemia, benign lymphocytosis, Niemann-Pick cells negative or weakly positive. Megaloblastic anemia, hemolytic anemia, aplastic anemia, etc., young red blood cells are negative reactions. Occasionally, individual young red blood cells are positive. 2, to help identify leukemia cells and adenocarcinoma bone marrow metastasis of adenocarcinoma cells, adenocarcinoma cells were positive. Tips: According to the nutritional status of the whole body, milk, eggs, fruits, soy milk, etc. are given in the meal. Normal value Normally, the erythrocyte system of protoplasts, immature red blood cells and mature red blood cells; the granulocyte system of myeloblasts, protomono cells and most lymphocytes are negative. Granulocytes and juvenile monocytes after the early granule stage may be weakly positive. Clinical significance 1. Acute lymphocytic leukemia, lymphoid malignant proliferative diseases, erythroleukemia, Gaucher disease, primordial cells are strongly positive or positive; iron deficiency anemia, globin production disorders, myelodysplastic syndrome can also be positive Response; acute myeloid leukemia, acute monocytic leukemia, benign lymphocytosis, Niemann-Pick cells negative or weakly positive. Megaloblastic anemia, hemolytic anemia, aplastic anemia, etc., young red blood cells are negative reactions. Occasionally, individual young red blood cells are positive. 2, to help identify atypical megakaryocytes and Hodgkin cells, megakaryocytes showed a strong positive reaction; Hodgkin cells showed a weak positive or negative reaction. 3, to help identify leukemia cells and adenocarcinoma bone marrow metastasis of adenocarcinoma cells, adenocarcinoma cells were positive. Precautions The criteria for judging vary from cell to cell and are generally divided into 5 levels, ie 0 to 4 levels. Inspection process 1. Freshly dried blood or bone marrow tablets are fixed in 95% ethanol for 10 min, rinsed several times with distilled water, and dried. 2, immersed in 10g / L aqueous solution of periodic acid for 10min, rinsed several times with distilled water, to be completely dry. 3. Put in Schiff liquid for 30 minutes, rinse with tap water for about 5 minutes, rinse with distilled water, then counter-dye with 20g/L methyl green for 20min, wash with water and dry. Not suitable for the crowd no. Adverse reactions and risks no.