Litmus milk test

The litmus milk test is based on the rich protein and sugar in the milk, and the ability of various bacteria to decompose these substances is different. Therefore, there are several different reactions to test the principle of bacteria. Clostridium typhimurium caused by Clostridium - tetanus; Clostridium perfringens - gas gangrene; Clostridium botulinum - botulism; C. difficile - pseudomembranous colitis. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: not fasting Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The type and proportion of the flora in the body are normal, and the human body is in a dynamic balance. Clinical significance Mainly used for the identification of Clostridium, Streptococcus and Propionibacterium. Abnormal result Clostridium typhimurium caused by Clostridium - tetanus; Clostridium perfringens - gas gangrene; Clostridium botulinum - botulism; C. difficile - pseudomembranous colitis. Streptococcus disease 1, group A streptococcus caused by diseases such as suppurative infections including local infections (such as lymphangitis, cellulitis, etc.) and other systemic infections (such as angina, otitis media, puerperal infections, etc.). Because the hyaluronidase produced can decompose connective tissue to loosen, streptokinase can dissolve fibrin, and the chain enzyme decomposes DNA to make the pus thin; therefore, the above invasive enzymes can help the spread of bacteria and cause diffuse inflammation. . Allergic diseases (hypersensitive diseases) include rheumatic fever, acute glomerulonephritis; scarlet fever caused by toxic toxins of rash disease. 2, type A hemolytic streptococcus can cause infective endocarditis. Propionibacterium is a common bacterium of the lung infection, causing a series of symptoms of pulmonary infection. The person in need of examination has the above symptoms. Precautions Inappropriate crowd: No. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process Litmus milk test 1. Inoculate the test bacteria in the culture solution to be inoculated in the litmus milk medium. If it is Clostridium spp., add sterile iron in the medium and incubate at 35 ° C for 18~24h. Can be extended to 14d. Observation results. 2. Results: (1) Acid production: Fermented lactose produces acid, which turns the indicator into pink. (2) Gas production: fermenting lactose while producing gas, can open the above Vaseline. (3) Coagulation: The casein in the milk is coagulated due to too much acid production. (4) Deuteration: The solidified casein is further hydrolyzed to hydrazine, the upper layer of the medium is cleared, and the casein which is not completely deuterated remains at the bottom. (5) Alkali production: Lactose is not fermented, due to decomposition of nitrogen-containing substances, amines and ammonia are formed, the medium becomes alkali, and the indicator turns blue. Not suitable for the crowd No. Adverse reactions and risks no.