Whole blood nickel (Ni)

The determination of whole blood nickel helps to estimate the extent of acute nickel poisoning. And it has certain diagnostic significance for diseases such as myocardial infarction and cerebrovascular accident. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Uremia, cirrhosis, long-term oral contraceptives, etc. Normal value: Whole blood nickel: 0.15-7.7μmol/L Above normal: Acute myocardial infarction (transient rise of nickel, peak serum concentration after 12 to 36 hours after onset), acute nickel poisoning, stroke, burns, acute hepatitis, sepsis, pregnancy poisoning, chronic kidney disease of hemodialysis. negative: Positive: Tips: After 8 pm on the day before the medical examination, you should start fasting for 12 hours, so as not to affect the test results. Normal value Spectrophotometry (AAS) is 0.15 to 7.7 μmol/L (0.9 to 44.5 μg/dl). Clinical significance 1. Increase acute myocardial infarction (transient rise of nickel, peak serum concentration after 12 to 36 hours after onset), acute nickel poisoning, stroke, burn, acute hepatitis, sepsis, pregnancy poisoning, chronic kidney disease of hemodialysis, etc. . 2, reduce uremia, cirrhosis, long-term oral contraceptives. High results may be diseases: nickel poisoning, acute myocardial infarction, nickel carbonyl poisoning precautions For allergies to nickel, it is best not to check. Inspection process 1. Methyl thymol blue colorimetric method (MTB): After mixing for 5 min, the absorbance was adjusted to 0 o'clock with a wavelength of 610 nm or a red filter, and the optical path was 1.0 cm, and the absorbance of each tube was read. 2, o-cresol 酞 complex ketone direct colorimetry: Mix well, after 5 min, use wavelength 575 nm or red filter, light path 1.0 cm colorimetric, adjust the absorbance to 0 point with blank tube, and read the absorbance of each tube separately. If the specimen is turbid, jaundice or hemolysis, a calibration test is required, that is, 0.05 ml of 5 g/LEGTA solution is added to each of the blank tube and the measuring tube, and the absorbance is measured again, and the difference in absorbance between the two readings is corrected for the absorbance. 3. Ethylenediaminetetraacetic acid disodium titration method: Mix well, the liquid in the tube is light red, quickly titrate with the disodium edetate solution until the solution is light blue, and record the amount of EDTA-Na2 (ml). 4. Determination of ionized nickel: The nickel electrode used in the commercial ion nickel analyzer uses a neutral carrier as the active material of the nickel electrode to form a polyvinyl chloride (PVC) electrode film. The life of the electrode is about half a year: the pH electrode is made of special glass. Capillary; the reference electrode is made of silver/silver chloride. The reagent formulations, reagent dosages, and operation methods of various types of ionized nickel analyzers are different. Generally, the following steps are required. This method takes a domestic ion nickel analyzer as an example. (1) Connect the power supply, the instrument first performs display and electronic circuit detection, and then the two-point slope calibration can be performed. (2) When the slope is set, the suction needle is immersed in the vial containing the low and high two-concentration slope calibration instrument, and the slope calibration liquid is sucked up, and then the instrument is “beeped” and then removed. The sample is pushed back to the original position and the instrument automatically performs the slope calibration. (3) After the calibration is passed, the sample measurement can be performed. 1 capillary blood measurement: mix the capillary blood, push the suction needle, remove the plug at both ends, attach the connector at one end, mount the other end of the connector on the sampling needle, press the “Measure” button until the sample is completely filled with the sample measurement chamber. After that, release the “Measure” button and the injection pump stops working. At this time, the sample in the sample measuring chamber should be inspected for bubbles. If there are air bubbles, please release the “Measure” button for 8 seconds, then press the “Measure” button to continue to suck the sample until the bubbles are eliminated. Remove the sample, wipe the sample and push it back in place, and display the measurement data and print the result after 8 s of the injection. 2 serum measurement process is measured with whole blood. (4) After the specimen is measured, the instrument is flushed with the pipeline and the sample is measured. After the rinse is completed, the next sample can be measured. (5) The instrument will enter the “sleep” state after 10 minutes of the last calibration or blood sample measurement. At this time, if blood sample measurement is required, a certain calibration must be performed first. If there is no blood sample measurement, the instrument automatically performs a one-point calibration every 30 minutes. 5. Same atomic absorption spectrophotometry. Not suitable for the crowd Allergic to nickel. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.