Inulin fermentation test

The inulin fermentation test is to infer the bacterial type by verifying whether it can ferment inulin. For example, most newly isolated S. pneumoniae can ferment inulin in the inulin fermentation test, while the type A hemolytic streptococcus does not break down inulin, so Can be used for differential diagnosis of both. Basic Information Specialist classification: Respiratory examination classification: pathogenic microbiological examination Applicable gender: whether men and women apply fasting: not fasting Tips: Before the check: taboo: pay attention to normal eating habits, pay attention to personal hygiene. Normal value The type and proportion of the flora in the body are normal, and the human body is in a state of dynamic balance and health. Clinical significance Abnormal results S. pneumoniae pneumonia often occurs suddenly, manifested as high fever, chills, severe pleural pain, cough and rust. 10% to 20% of patients can be accompanied by bacteremia during the high fever period. The pathological manifestation is mainly that there is a large amount of fibrin exudate in the initial alveolar, followed by red blood cells and white blood cells exuding into the alveoli, which eventually leads to consolidation of the lung tissue in the lesion. The lesion usually only affects a single lobe, so it is called lobar pneumonia. If antibiotic treatment is used early, it can prevent lung consolidation. Streptococcus pneumoniae can also invade other parts of the body, causing secondary pleurisy, otitis media, mastoiditis, endocarditis and purulent meningitis. Invasive streptococcus can cause infective endocarditis. The differential diagnosis of Streptococcus pneumoniae and Streptococcus aureus infection in the population to be examined or the clinical diagnosis of the doctor. Precautions Inappropriate crowd: No. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process 【material】 Streptococcus pneumoniae and Streptococcus aureus colonies, inulin fermentation tubes. 【method】 1. Pick up S. pneumoniae and Streptococcus aureus colonies, inoculate them in the inulin fermentation tube, and incubate at 37 ° C for 10-24 hours. 2. Take the cells in the culture medium of the tested part to be inoculated into the inulin fermentation tube and incubate at 37 ° C for 10-24 hours. 3. Observe the condition of the two bacteria in the test group and the control group after growth in the glucose tube, and determine whether the bromocresol purple color is discolored or not. Not suitable for the crowd no. Adverse reactions and risks no.