anti-hepatitis delta virus IgM antibody

HDV is a defective virus, which can be replicated by relying on HBsAg. It is characterized by simultaneous or overlapping infection of HDV and HBV. The main route of transmission is life contact, and it can also be transmitted through blood products, blood transfusion, injection and the like. Its laboratory tests are mainly to detect antigens and antibodies. Basic Information Specialist classification: Infectious disease examination and classification: liver function examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Positive indicates a recent infection of hepatitis D and an acute phase of infection. Tips: It should be light, eat more fruits and vegetables, mix food reasonably, pay attention to adequate nutrition. Normal value ELISA method: negative. (1) Visual method: brown yellow, yellow is positive, pale yellow is suspicious, colorless negative. (2) Colorimetric method: The light absorption value at a wavelength of 492 nm is measured, and the S/N value or the threshold value (cotoff value) is calculated, and the S/N value of the test sample is ≥ 2.1 or ≥ the threshold value is positive, and vice versa. Threshold = negative control A492 average × 2.1 Clinical significance Positive: recent infection of hepatitis D and acute infection. Positive IgM anti-HDV antibody indicates short-term or acute phase of HDV infection. If the fluctuation of antibody is a manifestation of chronic HDV infection, if it is combined with anti-HBcIgM test, it can be distinguished from HBV infection or overlapping infection, so it has important diagnostic value. Precautions HDV itself is not infected and can only be infected with HBsAg-positive people. HDVAg is highly antigenic and can stimulate the body to produce IgG and IgM antibodies. These antibodies are non-neutralizing antibodies and have no protective effect on the body. Inspection process (1) The anti-human IgM monoclonal antibody was diluted with 0.05 mol/L of a pH 9.5 to 9.6 carbonate buffer, coated with a polystyrene plate, 100 μl per well, and allowed to stand at 4 ° C overnight. (2) Wash 3 times with PBST (without staying in the middle) and pat dry. (3) Add 100 μl of 1:1000 diluted test serum and negative and positive control serum (negative control 3 wells, positive control 2 wells) to each well for 1 h at 37 °C. (4) Wash the plate with PBST under negative pressure for 3 times (the method is the same as above) and drain (the serum and waste liquid are pumped into the disinfection bottle to prevent contamination of the environment). (5) 50-100 μl of appropriately diluted HDAg (2 to 4 ELISA units) was added to each well at 45 ° C for 1 h. (6) After washing 3 times, 50 μl of HRP-anti-HDV IgG antibody (in PBS containing 1% calf serum) was added to each well at 42 to 45 ° C for 1 h. (7) After washing 3 times, add 50 μl of freshly prepared substrate solution and let it develop in the dark at room temperature for 15-30 min. When the positive control is yellow, add 1 drop (25 μl) of 2 mol/L H2SO4 to each well to stop the reaction. Not suitable for the crowd Those who do not have an indication for examination should not do this check. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2. Risk of infection: If you use an unclean needle, you may be at risk of infection.