peroxidase staining

Some bone marrow or blood cells contain peroxidase. The enzyme decomposes H2O2 to produce new ecological oxygen. The new eco-oxygen can oxidize colorless benzidine to benzidine blue, which turns into a brown-black compound and precipitates in the cytoplasm. The peroxidase in the blood cells decomposes H2O2, releasing the nascent oxygen, oxidizing the leuco benzidine to the blue benzidine, which further turns into a brown-black compound that settles in the cytoplasm. Basic Information Specialist classification: cardiovascular examination classification: biochemical examination Applicable gender: whether men and women apply fasting: not fasting Tips: When you take blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Normal value Normal population granulocytes were positive (cytoplasm contains blue-green or tan particles); monocytes were negative or weakly positive; young red blood cells, plasma cells, megakaryocytes, and lymphocytes were negative. Clinical significance It is meaningful for the diagnosis of leukemia. In addition to the negative or weak positive reaction of early myeloblasts, acute myeloid leukemia is positive in all other stages. Acute multigranular promyelocytic leukemia has a strong positive response to promyelocytic cells. The acute cells of acute monocytic leukemia are negative or weakly positive. Chronic granulocytes are strongly positive. Lymphocytic leukemia was negative. Histiocytic leukemia was negative. Positive results may be diseases: negative results of acute lymphocytic leukemia may be diseases: chronic granulocytic leukemia, acute lymphocytic leukemia considerations Before inspection: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. After inspection: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process The examination process: the blood volume is generally 5 mL, and specific staining is performed after blood drawing. Not suitable for the crowd no. Adverse reactions and risks no.