Proline

Proline is the basic unit of protein and is one of the 21 amino acids that make up human proteins. The amino acids are all amino acids except the amino acids. The amino acids constituting the protein molecule are all L-amino acids, but in recent years it has been confirmed that they can be isomeric as D-amino acids, and the specific mechanism has not been studied. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: No clinical significance. Normal value: Proline: 102-336μmol/L Above normal: Hereditary hyperprolineemia. negative: Positive: Tips: The patient enters the vegetarian diet for 2 to 3 days. Normal value 102 to 336 μmol/L. Clinical significance Abnormal results: Increase hereditary hyperprolineemia. Need to check the crowd: Patients with suspected cases of hereditary hyperprolineemia need to be diagnosed. High results may be diseases: Amino acid metabolism disease considerations Note before inspection: 1. The patient enters the vegetarian diet for 2 to 3 days. 2. Collagen intake in food affects the excretion of hydroxyproline in urine. Requirements for inspection: 1. Inform the doctor about the food intake in the last 2~3 days. Not suitable for people: There is no inappropriate crowd. Inspection process Take the reference solution or the test solution 200μL, add 20μL of the normal leucine internal standard solution, add 100μL of 0.1mol/L phenyl isothiocyanate acetonitrile solution, 100μL of 1mol/L triethylamine acetonitrile solution, mix, room temperature Place it for 1 h, add 400 μL of n-hexane, shake it, place it for 10 min, and take the lower layer solution. Precisely draw 1μL of the test solution and the reference solution after derivatization, determine the proline and internal standard peak area according to the above chromatographic conditions, calculate the ratio of proline area to internal standard peak area, and calculate the proline by internal standard method. concentration. Not suitable for the crowd no. Adverse reactions and risks no.