Indigo green (indole, cyanocyanine) test

The liver is one of the important excretory organs of the human body. Many endogenous substances such as bile acid, bilirubin, cholesterol, etc., as well as exogenous substances such as drugs, poisons, dyes, etc., can be properly metabolized in the liver. Hepatocytes are excreted to the bile. When the liver cells are damaged, the excretion function of the above substances is reduced. According to this, artificial synthetic pigment can be exogenously administered, and liver clearance and excretion ability can be measured as one of liver function test items. Basic Information Specialist classification: Digestive examination classification: liver function test Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: normal. Normal value: ICGR in 15min: 0-10% Above normal: Various early hepatobiliary diseases, chronic familial non-hemolytic jaundice (Rotor syndrome), total bile duct obstruction, obstructive jaundice caused by malignant tumors, etc. negative: Positive: Tips: For patients with obesity, edema, and ascites, the ICG dosage should be calculated according to the standard weight, otherwise it will be too large. Normal value The ICGR is 0-10% in 15min, and the retention rate can be increased by 0.2%-0.6% for every 5 years old. Clinical significance In the early stages of various hepatobiliary diseases, ICGR increases in the blood. The ICGR of chronic hepatitis is between 15% and 20%, the activity is higher, and the cirrhosis can reach 35%. The degree of hepatitis is related to the stage of the disease and the degree of jaundice. In the recovery period, the test can return to normal earlier. Fatty liver This test is mostly normal. If the ICG load is changed, the maximum elimination rate (ICGRmax) is calculated to help diagnose the underlying cirrhosis. ICGR depends on whether liver fibrosis occurs in liver cancer, and ICGR in normal liver fibrosis. Therefore, this test is used to determine the presence or absence of liver fibrosis in patients with liver cancer. Increased ICGR is also seen in chronic familial non-hemolytic jaundice (Rotor syndrome), obstructive jaundice, and obstructive jaundice caused by malignant tumors. This test is similar to the positive rate of the BSP test. In addition, it can also be used to measure liver blood flow and the like. High results may be diseases: cirrhosis precautions 1, the use of time should be accurate, serum should be separated in time after blood collection. ICG is sensitive, should avoid light, timely measurement, >6h can affect the results. 2. For patients with obesity, edema, and ascites, the ICG dosage should be calculated according to the standard weight, otherwise it will be too large. 3. Theoretically, it is estimated that the adult per kilogram of body weight is equivalent to 50 ml of circulating plasma, so it is injected at 0.5 mg/kg ICG. The ICG concentration in plasma at the time of zero injection is 0.5 mg/50 ml, that is, 10 mg/L. This is subject to the calculation of the ICG retention rate. 4. ICG has good stability in protein solution. When the ICG standard curve is used, an appropriate amount of serum is added to increase the stability of color development, and the result is consistent with serum. Inspection process 1. Calculate the dosage according to body weight, ie 0.5mg/kg. Diatnogreen injection, each 25mg, plus 5ml diluted with water for injection from one side of the elbow. 2, accurate recording time, 15ml from the contralateral elbow vein to take blood about 4ml, separation of serum, pay attention to non-hemolysis. 3, take 1ml of serum and 2ml of normal saline, 805nm wavelength, zero with distilled water, read absorbance of A1. Then add 1 drop of the bleaching agent, mix and immediately colorimetrically, and read the absorbance to A2. A1-A2 is the absorbance of serum ICG, and the standard curve is obtained to obtain the concentration of ICG. Not suitable for the crowd no. Adverse reactions and risks no.