Protein, amino acid decomposition product test

The protein and amino acid decomposition products test analyzes the metabolic products of bacteria and classifies the metabolic characteristics of bacteria. The metabolic pathway of bacteria includes two major categories of synthesis and decomposition. Bacterial anabolism is similar to eukaryotic bacteria, but its catabolism varies greatly depending on the bacterial enzyme system. Catabolism can be accompanied by the production of ATP and other forms of energy. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: not fasting Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The type and proportion of the flora in the body are normal, and the human body is in a dynamic balance. Clinical significance For the catabolic characteristics, determine which bacterial infection the patient is. Abnormal results of intestinal bacteria caused by pain, stomach acid, bloating, diarrhea, abdominal pain, falling, pus and bloody stools and other symptoms, severe cases can also cause gastrointestinal bleeding, perforation, induced cancer, such as stomach cancer, colon cancer and other symptoms. Clinical manifestations of Proteus infection: 1, gastroenteritis type incubation period 3 ~ 20h, onset of suddenness, nausea, vomiting, abdominal pain, diarrhea, diarrhea is watery, with mucus, foul odor, no pus, several times a day to more than ten times. After 1/3 to 1/2 of patients with gastrointestinal symptoms, fever accompanied by chills, and decreased after several hours. In severe cases, there is dehydration or shock. 2, allergic type incubation period 1/2 ~ 2h, manifested as systemic congestion, facial flushing, drunken appearance, itchiness around the body, mild gastrointestinal symptoms. A small number of patients can develop urticaria. Paratyphoid B has caused headache, nausea, vomiting symptoms, neck stiffness, lethargy and meningeal irritation. The people in need of examination need to have a headache, nausea, vomiting symptoms, neck stiffness, lethargy and meningeal irritation and other symptoms of paratyphoid B. Precautions Inappropriate crowd: No. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process 1. Indoltest: bacteria containing tryptophanase (such as Escherichia coli, Proteus, etc.) can decompose tryptophan to form hydrazine. If dimethylaminobenzaldehyde is added, it combines with hydrazine to form a rose.吲哚, red, said 吲哚 test positive. 2. Hydrogen sulfide test: Proteus and B. parahaemolyticus can decompose sulfur-containing amino acids such as cystine and methionine to form hydrogen sulfide. In the presence of lead acetate or ferrous sulfate, black lead sulphide or ferrous sulphide is formed, which can be used to identify bacteria. 3. Urea decomposition test: Proteus has urease, which can decompose urea to produce ammonia. The medium is alkaline, and phenol red is used as an indicator to detect red color, which is different from Salmonella. 吲哚 (I), methyl red (M), VP (V), citrate use (C) four tests, commonly used to identify enterobacteria, collectively referred to as the IMViC test. E. coli is "++--" and aerogenic bacillus is "--++". Gas phase and liquid chromatography can accurately and quickly determine the type of bacteria by detecting volatile or non-volatile organic acids and alcohols in bacterial catabolic products. It is a high-tech for biochemical identification of bacteria. Not suitable for the crowd no. Adverse reactions and risks no.