Cholinesterase

Cholinesterase (ChE) is secreted by the liver and secreted into the blood. Like plasma albumin, it is an indicator of the function of the liver to synthesize proteins. There are two types of ChE for humans and animals. One type is true cholinesterase (AChE), which is distributed in red blood cells and brain gray matter. The other type is cholinesterase (PChE), which is distributed in liver, white matter and serum. The main function of ChE is to catalyze the hydrolysis of acetylcholine. Commonly used colorimetric method and continuous monitoring method. Basic Information Specialist classification: Digestive examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Found in organophosphate poisoning, hepatitis, cirrhosis, malnutrition, pernicious anemia, acute infection, myocardial infarction, pulmonary infarction, muscle injury, chronic nephritis, dermatitis and late pregnancy, as well as intake of estrogen, cortisol, quinine, morphine , codeine, theobromine, aminophylline, barbital and other drugs. Normal value: Cholinesterase: 130-310U/L Above normal: Found in nervous system diseases, hyperthyroidism, diabetes, hypertension, bronchial asthma, type IV hyperlipoproteinemia, renal failure and so on. negative: Positive: Tips: Keep a normal mindset. Normal value Colorimetric method 130 ~ 310U / L; enzymatic children and adults male, female (40 years old or older) 5410 ~ 32000U / L; female (16 ~ 39 years old) 4300 ~ 11500U / L. Clinical significance Increased: seen in nervous system diseases, hyperthyroidism, diabetes, hypertension, bronchial asthma, type IV hyperlipoproteinemia, renal failure and so on. Reduction: seen in organophosphate poisoning, hepatitis, cirrhosis, malnutrition, pernicious anemia, acute infection, myocardial infarction, pulmonary infarction, muscle injury, chronic nephritis, dermatitis and late pregnancy, as well as intake of estrogen, cortisol, quinine , morphine, codeine, theobromine, aminophylline, barbital and other drugs. Low results may be diseases: organophosphorus pesticide poisoning, hepatitis, phosphorus poisoning, pediatric organophosphorus pesticide poisoning, pesticide poisoning, elderly fatty liver, cirrhosis of the elderly, fulminant hepatic failure, pyrethroid pesticide poisoning, fatty liver results High possible disease: cirrhosis considerations 1. The color development of this method is unstable, especially when the room temperature exceeds 20 °C, and the color of the ferric chloride is added. The color of the supernatant should be quickly filtered or centrifuged, otherwise the error is large, and when the room temperature is too high, It is best to operate and filter in ice water baths. 2, some patients with unclear filtrate turbidity, colorimetric difficulties, such phenomena are seen in liver abscess, sepsis, may be due to the high serum mucin content of such patients, trichloroacetic acid failed to completely precipitate protein, in some methods The addition of phosphoric acid can overcome this disadvantage. Inspection process ChE in serum hydrolyzes acetylcholine to choline and acetic acid, and unhydrolyzed residual acetylcholine reacts with hydroxylamine to form acetohydroxylamine. Acetylhydroxylamine and iron ion form a brown iron complex in acidic solution. The residual acetylcholine content is calculated according to the color depth. Enzymatic activity of hydrolyzing acetylcholine. Not suitable for the crowd no. Adverse reactions and risks no.