long carbon chain fatty acids

Fatty acids in the blood are mainly formed by esterification. About 45% of them are glycerol, 15% and CH, 35% with PL synthetic esters, and only 5% of fatty acids are in a free state, mainly long-chain fatty acids. Normal value of long carbon chain fatty acids: gas chromatography-mass spectrometry: 0.014% of total fatty acids. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: If the serum free fatty acid concentration is greater than 2mmol / L, it can be diluted after appropriate dilution. Normal value Gas chromatography-mass spectrometry accounted for 0.014% of total fatty acids. Clinical significance Elevation: Adrenal leukodystrophy (ALD). High results may be diseases: protein-energy malnutrition considerations If the serum free fatty acid concentration is greater than 2mmol / L, it can be diluted after appropriate dilution. Inspection process 1. Mixing reagents: (1) Reagent I: buffer solution I4.0ml, ATP2.0ml, MgCl22.0ml, coenzyme A2.0ml, TritonX-1004.0ml, acyl-CoA synthetase 2.0ml, plus double distilled water 4.5ml (20.5ml total) . (2) Reagent II: 10.0 ml of MES buffer, 6.0 ml of 4-aminoantipyrine, TBHB 8.0 ml, 2.0 ml of NaN3 solution, Triton X-1004.0 ml, peroxidase solution 0.4 ml, double distilled water 10.0 ml (Total 40.4ml). (3) Reagent III: 2.1 ml of NEM buffer and 2.0 ml of acyl-CoA oxidase (4.1 ml in total). The above three mixed reagents can be stable for 3 days at 4 ° C in the dark. 2. Add 1 ml of reagent and 2 ml of reagent II to each tube and mix at 37 ° C for 10 min. 3. Add 50 μl of blank and serum samples, mix at 37 ° C for 5 min, and measure the absorbance at 546 nm to A1. 4. Add 0.20 ml of reagent III and mix. After the reaction is stopped for 20-25 minutes, the absorbance is measured at 546 nm to be A2. Not suitable for the crowd no. Adverse reactions and risks no.