β1-pregnancy-specific protein

SP1 is a pregnancy-specific protein synthesized by the syncytium trophoblast. The electrophoresis position is in the serum β-globin band, and the half-life is 30-40 hours. It is a unique protein of pregnancy and can check the placental function. Basic Information Specialist classification: maternity check check classification: blood test Applicable gender: whether women are fasting: fasting Analysis results: Below normal: Serum levels are reduced before and after spontaneous abortion. Severe pregnancy-induced hypertension, intrauterine growth retardation and stillbirth (can be reduced to zero). Normal value: 1-1-pregnancy-specific protein (37 weeks pregnant): 150-246 μg/L Above normal: Elevated serum levels are common in trophoblastic diseases such as hydatidiform moles, invasive hydatidiform moles, malignant choriocarcinoma, malignant teratoma, and most twin pregnancies. In addition, tumors of certain malignant tumors such as testis, lung, ovary, breast and gastrointestinal tract may be slightly elevated. negative: Positive: Tips: Try to eat less and eat as much as possible, and arrange your diet reasonably. Normal value Immunoelectrophoresis 1, serum Weekly mean ± standard deviation (μg / L) 24 weeks: 61 ± 18 26 weeks: 86±23 28 weeks: 114±21 30 weeks: 130±30 32 weeks: 158±30 34 weeks: 163±41 36 weeks: 196±52 37 weeks: 198±48 41 weeks: 188±68 There was no significant difference between primipara and maternal. 2. The maternal SP1 level in urine full-term pregnancy reached the highest value of 30μg/L. 3, amniotic fluid pregnancy 40 weeks 1400μg / L 4, breast milk 3 weeks postpartum 160μg / L Clinical significance 1, elevated serum levels are common in trophoblastic diseases such as hydatidiform mole, invasive hydatidiform mole, malignant choriocarcinoma, malignant teratoma and most twin pregnancy can be significantly increased. In addition, tumors of certain malignant tumors such as testis, lung, ovary, breast and gastrointestinal tract may be slightly elevated. 2. Serum levels are reduced before and after spontaneous abortion. Severe pregnancy-induced hypertension, intrauterine growth retardation and stillbirth (can be reduced to zero). Low results may be diseases: high fetal growth restriction results may be diseases: considerations for twin pregnancy Physiological changes: The serum SP1 content can be measured 7 days after fertilization, and can be used as a diagnostic indicator for early pregnancy. SP1 has a long half-life in the blood, slow metabolism, and a small range of physiological fluctuations per day. Therefore, it can better reflect the function of the placenta during pregnancy, and can understand the survival status of the fetus through its level change. Decreased serum SP1 levels during pregnancy suggest a risk of miscarriage and have important reference value for the prognosis of threatened abortion. Some people think that serum SP1 level is related to placental weight. Serum SP1 and placental lactogen (hPL) can be used as a diagnostic index for multiple pregnancy. After delivery, serum SP1 levels rapidly decreased and disappeared on the 12th day after delivery. The appearance of urinary SP1 is later than serum, which can be measured after 3 weeks of gestation, and is less sensitive to the diagnosis of early pregnancy than urinary chorionic gonadotropin (HCG). Inspection process 1. Colorimetric method: operate as required. Mix well, within 10 ~ 30min, with a wavelength of 490nm, a diameter of 1cm, distilled water to zero colorimetric, with the difference of AU-AC, check the standard curve to obtain the enzyme activity unit. 2. Continuous monitoring method: (1) Take serum 0.05ml, add 2ml of NADH solution, and water bath at 37 °C for 10-15min to complete non-specific oxidation of NADH. (2) Add 0.1 ml of α-ketobutyric acid pre-warmed to 37 ° C, mix well, and immediately pour into a constant temperature cuvette at 37 ° C for monitoring. 340nm wavelength, 1cm optical path, air zero. (3) If ΔA/min>0.08, the serum is diluted 5 or 10 times with phosphate buffer and retested. Not suitable for the crowd no. Adverse reactions and risks no.