α-Teaphenol acetate staining

Α-naphthyl acetate esterase is a non-specific esterase in leukocytes, which can hydrolyze α-naphthol in matrix solution to produce α-naphthol, and then couple with diazonium salt to form gray-black insoluble precipitate, which is located in enzyme activity. Within the cytoplasm. Basic Information Specialist classification: Oncology examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Negative is seen in cells of multiple myeloma. Positive: Positive can be seen in cells such as monocytic leukemia, histiocytic leukemia, reticulum sarcoma, granulocyte-monocyte leukemia, red blood disease, erythroleukemia, promyelocytic leukemia, and the like. Tips: Before the examination, the diet is light and alcohol is prohibited. Check for an empty stomach in the morning. Normal value Proto-mononuclear cells, juvenile monocytes, monocytes, megakaryocytes, and platelets were positive reactions; granulocytes, erythrocytes, lymphocytes, and plasma cells were negative in each phase. Clinical significance Positive can be seen in cells such as monocytic leukemia, histiocytic leukemia, reticulum sarcoma, granulocyte-monocyte leukemia, red blood disease, erythroleukemia, promyelocytic leukemia, and the like. Negative is seen in cells of multiple myeloma. Positive results may be disease: monocytic leukemia results may be negative disease: multiple myeloma considerations If necessary, a specimen positive for α-naphthyl acetate esterase staining is added for sodium chloride inhibition test. Red blood disease, erythroleukemia, and promyelocytic leukemia are not inhibited by the sodium fluoride test and are still positive. Inspection process 1. Fixation: fresh smear is set with 10% formaldehyde saline for 5 min or formaldehyde vapor for 5-10 min. 2, rinse with running water for 5 min, dry. 3, into the working solution, 37 ° C 1h. 4. After washing with water, it was counterstained with methyl green aqueous solution for 5 min. 5, wash, dry after microscopic examination. The sodium fluoride inhibition test was used as a control, and 15 mg of sodium fluoride was added to 40 ml of the working solution, and the other methods were the same as above. After staining with two methods, 100 or 200 cells were counted by oil mirror, and the positive rate and integral before and after inhibition were calculated. Not suitable for the crowd no. Adverse reactions and risks no.