Optochin Sensitivity Test

The Optochin Sensitivity Test is a test method for the specific inhibition of Streptococcus pneumoniae by ethyl hydrogenated hydroxyquinine hydrochloride and the identification of Streptococcus pneumoniae and other streptococci with no effect on other Streptococcus. Its mechanism of action may be to interfere with folate biosynthesis. Basic Information Specialist classification: Respiratory examination classification: biochemical examination Applicable gender: whether men and women apply fasting: not fasting Tips: Maintain a normal diet and schedule. Normal value On the throat and tonsil mucosa, mainly streptococcus aureus and catarrhal bacteria predominate, in addition to the potential pathogenic microorganisms such as pneumococcal, influenza bacillus, streptococcus mutans and so on. As long as the type and proportion of the flora are normal, the human body is in a dynamic equilibrium. It is in the normal range. Clinical significance Mainly used for the identification of Streptococcus pneumoniae (sensitive) and other streptococcus (resistance). Abnormal results The disease caused by Streptococcus pneumoniae is lobar pneumonia, which can be secondary to pleurisy, empyema, otitis, meningitis, sepsis, etc. Other streptococci, such as hemolytic streptococcus, a systemic, allergic, inflammatory disease caused by infection with hemolytic streptococcus. Clinically, there are mainly arthritis, carditis, skin ring erythema, subcutaneous nodules and chorea; secondary manifestations include fever, sore throat, nodular erythema, and increased erythrocyte sedimentation rate. The people in need of examination have patients with suspected streptococcal infections. Positive results may be diseases: precautions for lobar pneumonia Inappropriate crowd: temporarily unknown. Forbidden before examination: Maintain a normal diet and schedule. Requirements for inspection: Actively cooperate with the doctor's work. Inspection process Optochin sensitivity test Method: The broth culture of the test bacteria was evenly spread on a blood agar plate with a cotton swab, and a piece of 5 μg of Optoxin paper was placed, and the candle was placed in a candle or carbon dioxide incubator, and incubated at 35 ° C for 18~ 24h, observe the results. Results: The diameter of the inhibition zone was more than 14 mm, which was sensitive, and less than or equal to 14 mm was negative. Not suitable for the crowd no. Adverse reactions and risks no.