C-reactive protein (CRP)

A protein that can bind to the pneumococcal cell wall C-polysaccharide (found in 1941), which is found in the serum of acute inflammatory patients, is named C-reactive protein (CRP). The first semi-quantitative precipitation test was used to prepare high-quality antiserum, which can establish a quantitative method with high sensitivity, high specificity and good repeatability. CRP is synthesized by hepatocytes and activates complement and promotes phagocytosis of granulocytes and macrophages. CRP is the first protein that is considered to be an acute phase response, which is normally present in very small amounts and has a sharp increase in blood levels during acute trauma and infection. CRP is the most commonly used indicator of acute phase response in the clinic. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Rare. Normal value: C-reactive protein: 800-8000μg/L Above normal: 1. Acute inflammation or tissue necrosis, such as severe trauma, surgery, acute infection, etc. CRP often rises sharply within a few hours, and rises before the erythrocyte sedimentation rate increases, CRP also returns to normal before the erythrocyte sedimentation rate; surgery The CRP concentration decreased 7-10 days after surgery, otherwise it suggested infection or complicated thrombosis; 2. Acute myocardial infarction increased 24 to 48 hours, decreased after 3 days, returned to normal after 1 to 2 weeks; 3. Acute rheumatic fever, rheumatoid Arthritis, systemic lupus erythematosus, bacterial infection, extensive tumor metastasis, active tuberculosis; 4. CRP does not increase when viral infection; 5.C-reactive protein can be used as an indicator of rheumatism, and predict myocardial infarction Relative risk. negative: Positive: Tips: After 8 pm on the day before the medical examination, you should start fasting for 12 hours, so as not to affect the test results. Normal value Which assay is used depends on the laboratory conditions and the sensitivity and specificity requirements. Immunodiffusion, radioimmunoassay, turbidity method, and enzyme-labeled immunoassay have practical value. Normal value 800-8000 μg / L (immuno diffusion or turbidity method). Clinical significance As a very sensitive indicator of acute phase response, plasma CRP concentration rapidly increased significantly in acute myocardial infarction, trauma, infection, inflammation, surgery, and tumor infiltration, up to 2000 times normal. Combined with clinical history, it helps to follow up the course of the disease. Especially in the process of inflammation, follow-up rheumatism, systemic lupus erythematosus, leukemia and so on. Raise 1. Acute inflammation or tissue necrosis, such as severe trauma, surgery, acute infection, etc. CRP often rises sharply within a few hours, and rises before the erythrocyte sedimentation rate increases. The recovery period CRP also returns to normal before the erythrocyte sedimentation rate; surgery The CRP concentration decreased 7-10 days after surgery, otherwise it may indicate infection or complicated thrombosis; 2, acute myocardial infarction increased 24 to 48 hours, decreased after 3 days, returned to normal after 1 to 2 weeks; 3, acute rheumatic fever, rheumatoid arthritis, systemic lupus erythematosus, bacterial infection, extensive tumor metastasis, active tuberculosis; 4. CRP does not increase when the virus is infected; 5, C-reactive protein can be used as an indicator of the condition of rheumatism, and predict the relative risk of myocardial infarction. High results may be diseases: pericardial incision syndrome, gout, pediatric skin mucosal lymph node syndrome, metabolic syndrome, systemic lupus erythematosus, amniocentesis syndrome, rheumatic chorea, pediatric nodular polyarteritis , pediatric aortitis, pediatric renal amyloidosis matters needing attention First, the precautions before blood draw 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, should pay attention after blood draw 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 3. Please inform the doctor about the recent medication and special physiological changes before the test. Inspection process 0.5 ml of CRP dilution was added to the test tube, and then 1 drop (50 ul) of serum was added. Mix well, add 10 ul of CRP reagent, mix and centrifuge (3000 rpm) for 1 min, and observe the results with a microscope. Those with clear agglutination were positive; those without agglutination were negative. 1. The reagent can be placed at 4~25°C, but it can't be frozen. Shake well when using. Strict operation should be carried out to prevent pollution as much as possible. For details, please refer to the kit instructions. 2, suitable for serum, chest, ascites, cerebrospinal fluid and other body fluids, but the specimen should not use anticoagulant anticoagulant. 3. The biggest advantage of the reagent is that it is fast and simple, the reagent is stable and easy to store, and the serum does not need to be inactivated and is not affected by RF interference. 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