5'-nucleotidase

The 5'-nucleotidase (5'-NT) is a hydrolase that is not specific for a substrate and can act on a variety of nucleotides. This enzyme is widely distributed in human tissues such as liver, gall bladder, intestine, brain, heart, and pancreas. Located in the plasma membrane of the cytoplasm, this enzyme is mainly found in the bile duct and sinusoidal membrane in the liver. The 5'-NT assay is mainly a colorimetric method, which is cumbersome to operate and difficult to automate, but the reagent is readily available and is suitable for use in a base unit. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: No clinical significance. Normal value: 5'-nucleotidase: 2-17U/L Above normal: Mainly found in hepatobiliary diseases, such as obstructive jaundice, liver cancer, hepatitis and so on. negative: Positive: Tips: 5'-NT assay is mainly used for the diagnosis of hepatobiliary diseases and the differential diagnosis of bone diseases. Normal value 2 to 17 U/L. Clinical significance The 5'-NT assay is mainly used for the diagnosis of hepatobiliary diseases and the differential diagnosis of bone diseases. Elevated serum 5'-NT activity is mainly seen in hepatobiliary diseases, such as obstructive jaundice, liver cancer, hepatitis, etc., and its activity changes are consistent with ALP. However, skeletal system diseases, such as tumor metastasis, malformation osteitis, rickets, hyperparathyroidism, etc., usually have elevated ALP activity, while 5'-NT is normal. Therefore, simultaneous determination of ALP and 5'-NT contributes to the differential diagnosis of hepatobiliary and skeletal diseases. High results may be diseases: cholestatic jaundice, liver cancer, hepatitis precautions 1. Determination by plasma can cause turbidity, and anticoagulants chelated with metal ions can interfere with the activation of magnesium. 2. Miteur can also be replaced by 1-amino-2-naphthol-4-sulfonic acid. 3. The enzymatic reaction of this method can also be used for 30 min, and the coefficient of the calculation result should be changed to 20. Inspection process 1. Follow the steps. 2. After mixing for 5 min, use 680 nm wavelength colorimetric, adjust to zero with distilled water, and read the absorbance of each tube. Not suitable for the crowd no. Adverse reactions and risks no.