5'-nucleotide phosphodiesterase isoenzyme

The 5'-nucleotide phosphodiesterase isoenzyme is a class of exonuclease. Four 5'-NPD active bands (I~IV) were isolated from normal serum by polyacrylamide electrophoresis, and V-band appeared under pathological conditions. The positive rate of 5'-NPD in primary hepatocellular carcinoma and metastatic liver cancer is above 80%. Normal value of 5'-nucleotide phosphodiesterase isoenzyme: four zones of I, II, III and IV can be seen by polyacrylamide electrophoresis, and there is no V zone. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Primary liver cancer (specific), hepatitis B surface antigen positive (partial). Tips: Determination of plasma can cause turbidity, and anticoagulants chelated with metal ions can interfere with the activation of magnesium. Normal value Polyacrylamide electrophoresis showed four zones of I, II, III and IV, and no V zone. Clinical significance Patients with primary liver cancer (specificity) and hepatitis B surface antigen positive in the V zone (partial). Positive results may be diseases: precautions for primary liver cancer 1. Determination by plasma can cause turbidity, and anticoagulants chelated with metal ions can interfere with the activation of magnesium. 2. Miteur can also be replaced by 1-amino-2-naphthol-4-sulfonic acid. 3. The enzymatic reaction of this method can also be used for 30 min, and the coefficient of the calculation result should be changed to 20. Inspection process Determined by polyacrylamide electrophoresis. Not suitable for the crowd no. Adverse reactions and risks no.