serum uric acid

Uric acid is the end product of sputum catabolism. Excreted by the kidneys with urine. The uric acid content of healthy adults is about 1.1g, about 15% of which is present in the blood. Most of the uric acid in the blood is reabsorbed by the renal tubules after being filtered by the glomerulus. Uric acid is one of the important components of non-protein nitrogen in plasma. In severe kidney damage, blood uric acid can be significantly increased. There is little change when it is slightly damaged. Therefore, blood uric acid measurement is a sensitive indicator for diagnosing kidney severity damage. Basic Information Specialist classification: urinary examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Less common, seen in pernicious anemia, celiac disease and so on. Normal value: Serum uric acid (uric acid enzyme-peroxidase coupling method): 90-420μmol/L Above normal: 1 acute and chronic nephritis blood uric acid increased significantly, and more significant than BUN, Cr, appeared earlier. In other advanced stages of kidney disease, blood uric acid such as kidney tuberculosis, pyelonephritis, and hydronephrosis also increased. Because of the greater influence of extrarenal factors, the degree of elevation is not directly proportional to the degree of impaired renal function. 2 gout, caused by nuclear protein and sputum metabolism disorder, blood uric acid> 595μmol / L. 3 leukemia, multiple myeloma, polycythemia vera, etc. due to increased metabolism of nucleic acid metabolism, increased blood uric acid. 4 other chloroform, carbon tetrachloride, lead poisoning, eclampsia, pregnancy reaction, strenuous exercise, etc. negative: Positive: Tips: Quality control serum should be measured simultaneously for each measurement. Normal value 1, phosphotungstic acid reduction method male 150 ~ 420μmol / L; female 90 ~ 357μmol / L. 2. Uricase-peroxidase coupling method is 90-420 μmol/L for adults. Clinical significance 1, blood uric acid increased: (1) Acute and chronic nephritis blood uric acid increased significantly, and was more significant than BUN and Cr, and appeared earlier. In other advanced stages of kidney disease, blood uric acid such as kidney tuberculosis, pyelonephritis, and hydronephrosis also increased. Because of the greater influence of extrarenal factors, the degree of elevation is not directly proportional to the degree of impaired renal function. (2) Gout, caused by nuclear protein and sputum metabolism disorder, blood uric acid>595μmol/L. (3) Leukemia, multiple myeloma, polycythemia vera, etc. due to increased metabolic breakdown of nucleic acids, blood uric acid increased. (4) Other chloroform, carbon tetrachloride, lead poisoning, eclampsia, pregnancy reaction, strenuous exercise, etc. 2, blood uric acid reduction: less common, seen in pernicious anemia, celiac disease and so on. High results may be diseases: gout, hyperuricemia, nephropathy, kidney stones, hyperuricemia considerations Fasting 8 hours before blood check. Inspection process 1, phosphotungstic acid reduction method: (1) In order to ensure accurate and reliable test results, each test should include quality control serum, and the value obtained should be within the allowable range. (2) (WHO recommended OCV is ±5%, RCV is ±10%. China's recommended RCV is 7.5%. (3) Red blood cells contain a variety of non-specific reducing substances, so serum or plasma is used as a sample. (4) Potassium oxalate reacts with phosphotungstic acid to form insoluble potassium phosphotungstate, which can cause turbidity of the color developing solution. Therefore, potassium oxalate cannot be used as an anticoagulant. (5) Serum and urine uric acid can be stable for 3 days at room temperature. After the urine is refrigerated, it can cause precipitation of urate precipitate. The solution is to adjust the pH of the urine to 7.5-8.0 and heat it to 50 ° C to completely dissolve the precipitate, and then measure. (6) The solubility of uric acid in water is extremely low. When preparing the standard solution, lithium carbonate is added to heat and dissolve. If there is no lithium carbonate, it can be replaced by potassium carbonate or sodium carbonate. (7) When the protein is precipitated with tungstic acid, part of the uric acid may precipitate together with the protein, and the amount of precipitation varies depending on the pH of the filtrate. If the filtrate pH is <3, the uric acid recovery rate is less than 90%, the pH value is 3.0 to 4.3, the recovery rate is 93% to 103%, the pH value is 2.4 to 2.7, and the recovery rate is 74% to 97%. (8) The quality of sodium tungstate should be guaranteed. If it contains molybdenum, it can react with phenolic substances in the blood and affect the measurement results. (10) The colorimetric time should be strictly controlled, and the colorimetric effect is best within 20 to 30 minutes at room temperature. 2. Uricase-peroxidase coupling method: (1) In order to ensure satisfactory quality, quality control serum should be measured at the same time for each measurement, and the obtained value should be within the allowable range. (2) Accuracy of the concentration of the standard solution and the accuracy of the amount of sample taken have a great influence on the measurement results. If the result tends to be high or low, the cause should be found from the standard solution and the standard amount of the sample. The glass equipment used should be clean and dry, and the accuracy of the pipette should be high. (3) The dry powder reagent is stored at 2 to 6 ° C for at least 6 months. The reconstituted reagent can be used for 6-8 hours at room temperature; it can be stabilized for 12-24 hours at 2-6 °C. If the dry powder is found to be agglomerated by moisture, or if it has a color, and the reconstitution does not match the measured value of the fixed serum, the reagent has been Deterioration should be discarded. (4) The final color reaction is a Trinder reaction, so it is greatly affected by the redox agent. When the reducing agent is contaminated, the result is significantly reduced or no color development. Contamination of the oxidant can result in higher results or reddening of the reagents. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally no complications.