Tiger red plate agglutination test

The tiger red plate agglutination test is used to diagnose brucellosis. Brucellosis is an acute and chronic infectious disease caused by Brucella. Infected by contact with sick animals or eating contaminated milk or dairy products. The incubation period is 1 to 3 weeks. The clinical features are slow onset, long-term fever, excessive sweating, weakness, systemic pain and joint pain. The symptoms in the acute phase are mostly resolved within 3 to 6 months. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normally negative. Positive: Positive (50%) agglutination, the coincidence rate of diagnosis of brucellosis was 95.92%. Tips: Shake the sample thoroughly before use. Do not use if there is any clot that is polluted or shaken. Normal value The normal value is negative. Clinical significance Abnormal result 1. Positive (50%) agglutination diagnosis Brucellosis coincidence rate was 95.92%. 2, suffering from acute infections such as streptococcus, typhoid, tuberculosis, influenza, malaria, etc., can also have a low positive reaction. Need to detect people Infection, often fever, sweating, weakness, and general pain. Positive results may be diseases: brucellosis, malaria, typhoid Before inspection: Shake the sample thoroughly before use. Do not use if there is any clot that is polluted or shaken. At the time of examination: the antigen and serum should be allowed to stand at room temperature for 30 to 60 minutes before being tested. Not suitable for the crowd: those who do not have an indication for examination should not do this check. Inspection process 1. Take 0.03ml of the tested serum and mix it with 0.03ml of antigen. Observe the results within 4 minutes. All the reactions above “+” are positive. Animals that have a positive response are required to undergo a complement fixation test or other complementary diagnostic test. 2. Brucellosis disease Red plate agglutination test antigen system is cultured in a suitable medium by using a Brucella strain with good antigenicity, and the cells are harvested, inactivated by heating, and then stained with tiger red dye solution after centrifugation. It is prepared by suspending in a lactic acid buffer. 3. Judging criteria for reaction intensity: “-” has no agglutination and is evenly pink. "+" can be slightly agglomerated, with a slight curling, and the liquid between the agglomerates is red. "++" forms a clear curl, and the liquid between the agglomerates is slightly clear. The "+++" agglutination reaction is stronger. The "++++" agglutination block is in the form of a mushroom cluster, and the liquid between the agglutination blocks is clear. Not suitable for the crowd Those who do not have an indication for examination should not do this check. Adverse reactions and risks Generally no complications and harm.